2AN0

Crystal Structure of the P332G mutant of the Bacillus subtilis NOS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	193
Detector technology	CCD
Collection date	2004-06-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.93
Spacegroup name	P 21 21 2
Unit cell lengths	80.971, 92.479, 62.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.22128
Rwork	0.218
R-free	0.25271
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.638
Phasing software	AMoRE
Refinement software	REFMAC (5.1.9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	86535
Completeness [%]	94.5	69.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG 4000, sodium Cacodylate, Potassium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	PEG 4000, sodium Cacodylate, Potassium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K