2AMY

X-Ray Structure of Human Phosphomannomutase 2 (PMM2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97243
Spacegroup name	P 32 2 1
Unit cell lengths	70.622, 70.622, 100.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.745 - 2.090
R-factor	0.20226
Rwork	0.199
R-free	0.27200
Structure solution method	MAD
RMSD bond length	0.022
RMSD bond angle	1.821
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.160
High resolution limit [Å]	2.090	2.090
Rmerge	0.069	0.599
Number of reflections	17547
<I/σ(I)>	20.356	3.031
Completeness [%]	99.0	91.5
Redundancy	12.3	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	10 MG/ML PROTEIN, 24 % W/V PEG 2K, 0.12 M GLYCINE, 0.1 M TRIETHANOLAMINE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	10 MG/ML PROTEIN, 24 % W/V PEG 2K, 0.12 M GLYCINE, 0.1 M TRIETHANOLAMINE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	10 MG/ML PROTEIN, 24 % W/V PEG 2K, 0.12 M GLYCINE, 0.1 M TRIETHANOLAMINE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	10 MG/ML PROTEIN, 24 % W/V PEG 2K, 0.12 M GLYCINE, 0.1 M TRIETHANOLAMINE, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K