2AMS
Structure of the oxidized Hipip from thermochromatium tepidum at 1.4 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-07-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.460, 46.550, 59.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.550 - 1.400 |
| R-factor | 0.1572 |
| Rwork | 0.157 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iua |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.200 |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | MOLREP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.550 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.061 | 0.190 |
| Number of reflections | 11907 | |
| <I/σ(I)> | 17.5 | 2.4 |
| Completeness [%] | 87.8 | 14.1 |
| Redundancy | 4.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | ammonium sulfate, sodium citrate, NaCl, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
