2AMM
Crystal structure of L122V/L132V mutant of nitrophorin 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-07-31 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.1808 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 34.147, 34.147, 256.223 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 1.900 |
| R-factor | 0.14629 |
| Rwork | 0.142 |
| R-free | 0.22125 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1pee |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.814 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 12989 | |
| <I/σ(I)> | 11.3 | 10.6 |
| Completeness [%] | 84.0 | 49 |
| Redundancy | 3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
