2AM3

Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with UDP-Glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-13
Detector	SBC-3
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	40.628, 82.492, 102.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.100 - 1.800
Rwork	0.186
R-free	0.22600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fo9 1foa
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.100	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	26941
Completeness [%]	82.5	78.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	40% PEG 6000, 1M Tris, MnCl2, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K