2ALM
Crystal structure analysis of a mutant beta-ketoacyl-[acyl carrier protein] synthase II from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.01259 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.393, 88.820, 61.048 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.229 |
| Rwork | 0.195 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ox0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.316 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.080 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.111 | 0.472 |
| Number of reflections | 10534 | |
| <I/σ(I)> | 5.7 | 1.6 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 20% PEG 3350, 0.2M Potassium acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
