2ALG
Crystal structure of peach Pru p3, the prototypic member of the family of plant non-specific lipid transfer protein pan-allergens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.200 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 102.378, 102.378, 77.295 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.190 - 2.300 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.26200 |
| Structure solution method | Anomalous Data |
| Starting model (for MR) | PDB 1FK5 Modelling model built with Swiss-Model server |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.190 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.133 | 0.422 |
| Number of reflections | 9797 | |
| <I/σ(I)> | 15.8 | 4.5 |
| Completeness [%] | 89.2 | 91.8 |
| Redundancy | 10.3 | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG monomethylether 5000, Mes, Ammonium Sulphate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
