2AL6

FERM domain of Focal Adhesion Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-04
Detector	MARRESEARCH
Wavelength(s)	1.12714
Spacegroup name	P 21 21 21
Unit cell lengths	50.540, 123.990, 133.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.350
R-factor	0.2164
Rwork	0.215
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aeh
RMSD bond length	0.007
RMSD bond angle	1.137
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.450
High resolution limit [Å]	2.350	2.350
Number of reflections	35211
Completeness [%]	98.1	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	PEG 4000, citrate, ammonium acetate, glycerol, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K