2AL1
Crystal Structure Analysis of Enolase Mg Subunit Complex at pH 8.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-10-22 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.000, 65.000, 85.900 |
| Unit cell angles | 90.00, 99.50, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.1531 |
| Rwork | 0.153 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 120985 | |
| Completeness [%] | 96.9 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 8 | 293 | PEG 8000, potassium chloride, pH 8.0, batch, temperature 293K |
