2AKP
Hsp90 Delta24-N210 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-11-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 90.500, 52.950, 84.240 |
| Unit cell angles | 90.00, 122.05, 90.00 |
Refinement procedure
| Resolution | 14.970 - 1.940 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ah6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.060 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 22195 | |
| Completeness [%] | 88.2 | 85.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 20% PEG8000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
