2AKO
Crystal structure of Glutamate 5-kinase from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 98.597, 99.409, 124.547 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.18005 |
| Rwork | 0.178 |
| R-free | 0.22130 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.208 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.103 | 0.326 |
| Number of reflections | 59989 | |
| <I/σ(I)> | 10.4 | 3.2 |
| Completeness [%] | 96.1 | 89.6 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 23% PEG 3350, .1M Hepes 7.5, .2M NaMalonate, Cryo 30% sucrose, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
