2AKF
Crystal structure of the coiled-coil domain of coronin 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9004 |
| Spacegroup name | P 1 |
| Unit cell lengths | 23.587, 23.569, 46.402 |
| Unit cell angles | 92.51, 96.85, 119.63 |
Refinement procedure
| Resolution | 20.000 - 1.200 |
| R-factor | 0.162 |
| Rwork | 0.160 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.541 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.250 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 24694 | |
| <I/σ(I)> | 13.1 | 7.1 |
| Completeness [%] | 92.4 | 81.6 |
| Redundancy | 3.74 | 2.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | MES, zinc sulfate, PEG MME 550, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
