2AKE

Structure of human tryptophanyl-tRNA synthetase in complex with tRNA(Trp)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-01
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	P 43 2 2
Unit cell lengths	133.200, 133.200, 138.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.970 - 3.100
Rwork	0.230
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o5t
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.290
High resolution limit [Å]	3.100	3.100
Rmerge	0.128	0.568
Number of reflections	23201
<I/σ(I)>	6.7	3
Completeness [%]	100.0	100
Redundancy	5.9	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	ammonium sulfate
2	1	2	ammonium sulfate