2AKA

Structure of the nucleotide-free myosin II motor domain from Dictyostelium discoideum fused to the GTPase domain of dynamin 1 from Rattus norvegicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-09-14
Detector	ADSC QUANTUM 4
Wavelength(s)	0.93927
Spacegroup name	P 21 21 21
Unit cell lengths	57.420, 126.990, 160.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.930 - 1.900
R-factor	0.185
Rwork	0.185
R-free	0.22400
Structure solution method	SIRAS
RMSD bond length	0.017
RMSD bond angle	1.700
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.098
Number of reflections	92665
<I/σ(I)>	18.1
Completeness [%]	99.1	94.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	Tris, PEG 8000, MPD, DTT, magnesium chloride, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K