2AJV
Crystal Structure of Cocaine catalytic Antibody 7A1 Fab' in Complex with Cocaine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0688 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.254, 117.320, 85.217 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.220 - 1.500 |
| R-factor | 0.18407 |
| Rwork | 0.182 |
| R-free | 0.22684 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ajs |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.571 |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.052 | 0.660 |
| Number of reflections | 72094 | |
| Completeness [%] | 99.5 | 97.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | PEG 4000, ammonium formate, sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
