2AJT
Crystal structure of L-Arabinose Isomerase from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-11 |
| Detector | ADSC QUANTAM Q315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 116.539, 116.539, 214.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.840 - 2.600 |
| R-factor | 0.2203 |
| Rwork | 0.217 |
| R-free | 0.27796 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.287 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.840 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.076 | 0.600 |
| Number of reflections | 44290 | |
| <I/σ(I)> | 16.3 | 2 |
| Completeness [%] | 98.3 | |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, Trisodium citrate dihydrate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
