2AJP
Crystal structure of a human pyridoxal kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97972 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 92.132, 114.971, 169.261 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.078 - 2.500 |
| Rwork | 0.241 |
| R-free | 0.28340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rfv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.411 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
| Rmerge | 0.121 | 0.050 | 0.670 |
| Number of reflections | 38925 | ||
| Completeness [%] | 97.8 | ||
| Redundancy | 6.7 | 6.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 9.5 | 291 | 30% PEG550MME, pH 9.5, vapor diffusion, temperature 291K |
