2AJG
Crystal structure of the editing domain of E. coli leucyl-tRNA synthetase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-02-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 111.489, 111.489, 136.764 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.21737 |
| Rwork | 0.216 |
| R-free | 0.24871 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.406 |
| Number of reflections | 33890 | |
| <I/σ(I)> | 7.8 | 1.7 |
| Completeness [%] | 98.6 | 98.3 |
| Redundancy | 5.3 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | (NH4)2SO4, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
