2AJA

X-Ray structure of an ankyrin repeat family protein Q5ZSV0 from Legionella pneumophila. Northeast Structural Genomics Consortium target LgR21.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-12
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	79.610, 90.457, 118.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.920 - 2.800
R-factor	0.221
Rwork	0.221
R-free	0.28600
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Completeness [%]	99.9	99.9
Redundancy	4.95

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2M potassium thiocyanate, 20% PEG 3350, 20 mM EDTA, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K