2AJ7

Crystal structure of a putative contractile protein (bh3618) from bacillus halodurans at 1.67 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-18
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000001
Spacegroup name	P 63
Unit cell lengths	108.810, 108.810, 82.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.120 - 1.670
Rwork	0.167
R-free	0.19100
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.597
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXD
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.140		1.730
High resolution limit [Å]	1.670	3.590	1.670
Rmerge	0.065	0.038	0.753
Number of reflections	64155	12693	10376
<I/σ(I)>	12.93	42.09	1.72
Completeness [%]	93.4		81.4
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP	7.3	277	0.2M KFormate, 20.9% PEG-3350, No Buffer, pH 7.3, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K
2	VAPOR DIFFUSION, SITTING DROP, NANODROP	7.1	273	0.2M NaF, 20.0% PEG-3350, No Buffer, pH 7.1, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 273K