2AHZ
K+ complex of the NaK Channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-19 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 82.203, 85.541, 129.608 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ahy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.051 | 0.474 |
| Number of reflections | 11424 | |
| <I/σ(I)> | 39 | 2.7 |
| Completeness [%] | 98.7 | 94.3 |
| Redundancy | 8.8 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 293 | PEG 400, KCl, CaCl2, Tris.HCl, t-butanol, n-Decyl-beta-D-maltopyranoside, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293 K, pH 8.00 |
