2AHA
Crystal structure analysis of a rate-enhanced variant of redox-sensitive green fluorescent protein in the reduced form, roGFP1-R8.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2004-08-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.693, 79.693, 166.779 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 1.980 |
| R-factor | 0.204 |
| Rwork | 0.199 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ema |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.141 | 0.483 |
| Number of reflections | 45355 | |
| <I/σ(I)> | 50.7 | |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 10.4 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 295 | sodium phosphate/citrate, ammonium sulfate, DTT, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
