2AH5
Hydrolase, haloacid dehalogenase-like family protein SP0104 from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2005-04-26 |
| Detector | SBC-1 |
| Wavelength(s) | 0.9792348 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.676, 46.653, 130.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.090 - 1.740 |
| R-factor | 0.19773 |
| Rwork | 0.196 |
| R-free | 0.22934 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.351 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.090 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 20861 | |
| Completeness [%] | 90.6 | 68.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 20 mM HEPES, 200mM NaCL and 1mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
