2AGQ

Fidelity of Dpo4: effect of metal ions, nucleotide selection and pyrophosphorolysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2004-12-29
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 2
Unit cell lengths	98.101, 103.009, 52.399
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.790 - 2.100
R-factor	0.227
Rwork	0.227
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jx4
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.060	0.406
Number of reflections	30510
<I/σ(I)>	13.9	1.49
Completeness [%]	95.1	87.6
Redundancy	2.44	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		pH 7.0