2AGK
Structure of S. cerevisiae His6 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-09-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.863, 71.427, 40.570 |
| Unit cell angles | 90.00, 104.71, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.300 |
| Rwork | 0.191 |
| R-free | 0.21600 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.117 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.300 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.050 | 0.639 |
| Number of reflections | 270881 | 7572 |
| <I/σ(I)> | 18.89 | 2.85 |
| Completeness [%] | 92.4 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG4000, sodium citrate, MgCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
