2AG6
Crystal structure of p-bromo-l-phenylalanine-tRNA sythetase in complex with p-bromo-l-phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 102.719, 102.719, 70.598 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.550 - 1.900 |
| R-factor | 0.21137 |
| Rwork | 0.209 |
| R-free | 0.24736 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1zh6 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.511 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | 0.741 |
| <I/σ(I)> | 39.9 | 4.75 |
| Completeness [%] | 98.3 | |
| Redundancy | 5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 20% PEG-300, %% PEG-8000, 10% GLYCEROL, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
