2AFH

Crystal Structure of Nucleotide-Free Av2-Av1 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-27
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	170.911, 75.893, 223.665
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.280 - 2.100
R-factor	0.172
Rwork	0.172
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m1n 2nip
RMSD bond length	0.013
RMSD bond angle	2.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.300	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	162611
Completeness [%]	95.7	89.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	PEG 10000, Tris, sodium chloride, magnesium chloride, dithionite, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298 K