2AEX
The 1.58A Crystal Structure of Human Coproporphyrinogen Oxidase Reveals the Structural Basis of Hereditary Coproporphyria
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9537 0.9795 0.9796 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 112.741, 112.741, 112.741 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.310 - 1.580 |
| R-factor | 0.18709 |
| Rwork | 0.186 |
| R-free | 0.20755 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.351 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.620 |
| High resolution limit [Å] | 1.500 | 1.580 |
| Number of reflections | 65081 | |
| Completeness [%] | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | MPD, citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
