2AEW
A model for growth hormone receptor activation based on subunit rotation within a receptor dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.720, 112.167, 93.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.700 |
| R-factor | 0.262 |
| Rwork | 0.257 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ghr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.082 | 0.324 |
| Number of reflections | 14626 | |
| <I/σ(I)> | 28 | 2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 70 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 283 | PEG, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
