2AEE
Crystal structure of Orotate phosphoribosyltransferase from Streptococcus pyogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-15 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 118.131, 118.131, 72.185 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.19131 |
| Rwork | 0.189 |
| R-free | 0.22942 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.649 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.157 | 0.667 |
| Number of reflections | 37510 | |
| <I/σ(I)> | 32.8 | 2.62 |
| Completeness [%] | 98.9 | 89.7 |
| Redundancy | 31 | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | Tris-Cl, Ammonium sulfate, Lithium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
