2AE8
Crystal Structure of Imidazoleglycerol-phosphate Dehydratase from Staphylococcus aureus subsp. aureus N315
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 4 |
| Unit cell lengths | 129.247, 129.247, 180.143 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.800 - 2.010 |
| R-factor | 0.19164 |
| Rwork | 0.188 |
| R-free | 0.22630 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.241 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.800 | 2.080 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.090 | 0.565 |
| Number of reflections | 97376 | |
| <I/σ(I)> | 10.3 | 3 |
| Completeness [%] | 99.5 | 96.2 |
| Redundancy | 7.4 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | PEG400, sodium citrate, Tris pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
