2ADY
Structural Basis of DNA Recognition by p53 Tetramers (complex IV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-05 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.997, 67.903, 74.989 |
| Unit cell angles | 90.00, 92.97, 90.00 |
Refinement procedure
| Resolution | 37.530 - 2.500 |
| R-factor | 0.169 |
| Rwork | 0.164 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tsr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.999 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.114 | 0.443 |
| Number of reflections | 15517 | |
| <I/σ(I)> | 12.8 | 2.9 |
| Completeness [%] | 96.2 | 98.4 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | Ammonium Fluoride, PEG 3350, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Fluoride | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Ammonium Fluoride | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | H2O |
