2ADJ
Crystal structure of monoclonal anti-CD4 antibody Q425 in complex with Calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 97.224, 97.224, 110.976 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9.980 - 2.900 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2adg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 3.030 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 10984 | |
| <I/σ(I)> | 17.1 | |
| Completeness [%] | 92.0 | 82.8 |
| Redundancy | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG4000, Sodium Acetate, Calcium, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0 K |
