2ADG

Crystal structure of monoclonal anti-CD4 antibody Q425

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1994-01-20
Detector	XUONG-HAMLIN MULTIWIRE
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	97.715, 97.715, 112.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.203
Rwork	0.203
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fvd
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	XUONG-HAMLIN ((DETECTOR SYSTEM))
Data scaling software	CCP4 ((AGROVATA)
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.610
High resolution limit [Å]	2.500	2.500
Number of reflections	18319
<I/σ(I)>	7.3
Completeness [%]	94.4	78.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	PEG4000, Sodium Acetate, EDTA, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K