2ACY

ACYL-PHOSPHATASE (COMMON TYPE) FROM BOVINE TESTIS

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1995-08
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	63.200, 36.200, 45.300
Unit cell angles	90.00, 104.10, 90.00

Refinement procedure

Resolution	15.000 - 1.800
R-factor	0.17
Rwork	0.179
R-free	0.23500 *
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	16.900 *
Data reduction software	MARXDS
Data scaling software	MARSCALE
Phasing software	MLPHARE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.059	0.215
Total number of observations	41285 *
Number of reflections	8949
<I/σ(I)>	36.98	10.52
Completeness [%]	98.8	92.4
Redundancy	4.6	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	3.5	4 *	PROTEIN WAS CRYSTALLIZED FROM 30% PEG8000 OR 4000, 25 MM ACETATE BUFFER PH 3.5, 0.2 M AMMONIUM-SULFATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	acetate	25 (mM)
2	1	reservoir	PEG4000	30 (%)
3	1	reservoir	ammonium sulfate	0.2 (M)