2ACW

Crystal Structure of Medicago truncatula UGT71G1 complexed with UDP-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2005-04-08
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	53.523, 90.662, 101.581
Unit cell angles	90.00, 102.67, 90.00

Refinement procedure

Resolution	99.100 - 2.600
R-factor	0.259
Rwork	0.205
R-free	0.28000
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.059	0.442
Number of reflections	29545
<I/σ(I)>	12.1	2.3
Completeness [%]	99.8	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG 3350, ammonium acetate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K