2ACP
Crystal structure of nitrophorin 2 aqua complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-08-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 39.754, 125.642, 33.468 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.1872 |
| Rwork | 0.185 |
| R-free | 0.23279 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1euo |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.028 |
| Data reduction software | XDISPLAYF |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 32453 | |
| <I/σ(I)> | 25 | 2 |
| Completeness [%] | 95.4 | 98 |
| Redundancy | 7.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | sodium citrate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
