2ACH

CRYSTAL STRUCTURE OF CLEAVED HUMAN ALPHA1-ANTICHYMOTRYPSIN AT 2.7 ANGSTROMS RESOLUTION AND ITS COMPARISON WITH OTHER SERPINS

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	75.300, 75.300, 208.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.700
R-factor	0.181 *
Rwork	0.181
RMSD bond length	0.011
RMSD bond angle	1.680
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.700 *
Rmerge	0.098 *
Total number of observations	61161 *
Number of reflections	14208 *
Completeness [%]	79.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium potassium phosphate	2.7 (M)
2	1	drop	protein	7 (mg/ml)
3	1	drop	precipitant	0.003 (ml)
4	1	drop	phosphate	5 (mM)