2AC3
Structure of human Mnk2 Kinase Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-02 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 104.502, 104.502, 72.351 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 2.100 |
R-factor | 0.21714 |
Rwork | 0.215 |
R-free | 0.25444 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.207 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 31011 | |
Completeness [%] | 99.5 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | salt, pH 7, VAPOR DIFFUSION, temperature 293K |