2ABK

REFINEMENT OF THE NATIVE STRUCTURE OF ENDONUCLEASE III TO A RESOLUTION OF 1.85 ANGSTROM

Replaces:  1ABK

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-05-18
Detector	XENTRONICS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	48.500, 65.800, 86.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.850
R-factor	0.185
Rwork	0.185
R-free	0.21600
RMSD bond length	0.015
RMSD bond angle	21.000 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.850
Rmerge	0.070
Number of reflections	27787
Completeness [%]	99.8
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	HEPES	5 (mM)
2	1	1		150 (mM)
3	1	1	glycerol	5 (%)