2AB1

X-Ray Structure of Gene Product from Homo Sapiens HS.95870

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-13
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	45.912, 57.731, 89.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.450 - 2.590
R-factor	0.197
Rwork	0.193
R-free	0.25700
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.644
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (refmac_5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.660
High resolution limit [Å]	2.600	2.600
Rmerge	0.106	0.363
Number of reflections	7727
<I/σ(I)>	10.348	2.748
Completeness [%]	99.1	91.6
Redundancy	7.4	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4	293	10 MG/ML PROTEIN, 2.1 M AMMONIUM SULFATE, 0.100 M SODIUM SUCCINATE, vapor diffusion, hanging drop, temperature 293K, pH 4.00