2AAT

2.8-ANGSTROMS-RESOLUTION CRYSTAL STRUCTURE OF AN ACTIVE-SITE MUTANT OF ASPARTATE AMINOTRANSFERASE FROM ESCHERICHIA COLI

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	156.800, 86.740, 79.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.22
RMSD bond length	0.009
RMSD bond angle	0.071
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Number of reflections	8783 *
Completeness [%]	64.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein solution	5.2 (mg/mL)
2	1	1	potassium phosphate	50 (mM)
3	1	1	ammonium sulfate	53 (%sat)