2AAA

CALCIUM BINDING IN ALPHA-AMYLASES: AN X-RAY DIFFRACTION STUDY AT 2.1 ANGSTROMS RESOLUTION OF TWO ENZYMES FROM ASPERGILLUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Detector technology	AREA DETECTOR
Detector	SIEMENS-XENTRONICS
Spacegroup name	C 2 2 21
Unit cell lengths	81.100, 98.300, 138.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 * - 2.100 *
R-factor	0.169 *
Rwork	0.112
Structure solution method	MOLECULAR REPLACEMENT
Refinement software	Servalcat (0.4.105)

Data quality characteristics

	Overall
High resolution limit [Å]	2.100
Rmerge	0.195 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *			PEG 8000, PH 3.0-4.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG8000