2AA4
Crystal structure of Escherichia coli putative N-ACETYLMANNOSAMINE KINASE, New York Structural Genomics Consortium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 87 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.991, 75.841, 166.646 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.18044 |
| Rwork | 0.179 |
| R-free | 0.24298 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.026 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.057 | 0.231 |
| Number of reflections | 26544 | |
| <I/σ(I)> | 12.8 | 3.9 |
| Completeness [%] | 97.0 | 87.3 |
| Redundancy | 13.6 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 290 | 30% PEG 1500, pH 7.20, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
