2A9U

Structure of the N-terminal domain of Human Ubiquitin carboxyl-terminal hydrolase 8 (USP8)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-01
Detector	SBC-3
Wavelength(s)	0.97883
Spacegroup name	P 21 21 21
Unit cell lengths	34.682, 64.820, 151.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.810 - 2.100
R-factor	0.21091
Rwork	0.208
R-free	0.25943
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.342
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.810	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.094	0.444
Number of reflections	18987
<I/σ(I)>	16.6	3.15
Completeness [%]	90.5	80.2
Redundancy	6.5	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	27% PEG3350, 0.2 M Li2SO4, 0.1 M bisTris, pH 5.6, 1 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298 K