2A8R

2.45 Angstrom Crystal Structure of the Complex Between the Nuclear SnoRNA Decapping Nudix Hydrolase X29 and Manganese in the Presence of 7-methyl-GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-04-14
Detector	RIGAKU RAXIS IIC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	50.200, 82.150, 112.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.000 - 2.450
Rwork	0.222
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u20
RMSD bond length	0.018
RMSD bond angle	1.222
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.510	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.046	0.365
Number of reflections	17613
<I/σ(I)>	14.1	3.1
Completeness [%]	99.4	99.5
Redundancy	3.46	3.48

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.68	293	4-5 mg/ml X29, 0.025M HEPES pH 7.68, 3/75% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K