2A71
Crystal structure of Emp47p carbohydrate recognition domain (CRD), orthorhombic crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.590, 129.620, 170.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.201 |
| Rwork | 0.198 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a6z |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.332 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.105 | 0.420 |
| Number of reflections | 22129 | |
| <I/σ(I)> | 8.7 | 4.8 |
| Completeness [%] | 87.7 | 90.9 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | PEG4000, Ammonium acetate, Sodium cacodylate, Calcuim chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
