2A70
Crystal structure of Emp47p carbohydrate recognition domain (CRD), monoclinic crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.620, 65.210, 72.460 |
| Unit cell angles | 90.00, 96.66, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.100 |
| R-factor | 0.135 |
| Rwork | 0.135 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a6z |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.031 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.090 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.065 | 0.380 |
| Number of reflections | 176286 | |
| <I/σ(I)> | 8.9 | 2.3 |
| Completeness [%] | 98.5 | 85.6 |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 283 | PEG3350, Potassium acetate, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
