2A6V

Crystal structure of Emp46p carbohydrate recognition domain (CRD), potassium-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-01-21
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	54.320, 55.890, 77.470
Unit cell angles	90.00, 108.28, 90.00

Refinement procedure

Resolution	20.000 - 1.520
R-factor	0.191
Rwork	0.189
R-free	0.21800
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.404
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.570
High resolution limit [Å]	1.520	1.520
Rmerge	0.039	0.328
Number of reflections	67798
<I/σ(I)>	13.3	3.6
Completeness [%]	99.6	99.9
Redundancy	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	PEG3350, Potassium fluoride, HEPES, Ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	PEG3350, Potassium fluoride, HEPES, Ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K