2A6S
Crystal structure of YoeB under isopropanol condition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.974, 60.989, 98.560 |
| Unit cell angles | 90.00, 98.03, 90.00 |
Refinement procedure
| Resolution | 19.960 - 1.770 |
| R-factor | 0.196 |
| Rwork | 0.195 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | extracted from YoeB structure from 2A6Q |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.800 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Number of reflections | 33168 | |
| <I/σ(I)> | 42.01 | |
| Completeness [%] | 99.7 | 97.5 |
| Redundancy | 7.4 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277.2 | Tris-HCl, CH3COONH4, isopropanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.2K |
